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When demultiplexing, the final sample sheet offered towards the FASTQ generation software really should provide the i5 index in the right orientation for that platform. However, most Illumina sample sheet era application handles this automatically.
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The program quickly produces the reverse complement as wanted for analysis. In the event the sequence is reverse complemented, this will likely only be revealed during the SampleSheetUsed.csv file.
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Index 2: the i5 index column is from the reverse enhance orientation (are definitely the reverse complement of the i5 indexes from the sample sheet).
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When assessing index sets for colour harmony, the sequence noticed by the instrument has to be assessed, whatever the orientation utilized for the sample sheet. With the i7 index, often asses get more info coloration balance using the "i7 bases for sample sheet" column orientation.
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